Composite

Part:BBa_K2285015:Design

Designed by: Zhuoyang Chen   Group: iGEM17_SJTU-BioX-Shanghai   (2017-10-18)


J23119+Target3+amilCP(RBS+amilCP+Term)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Target3 with the downstream reporter gene is hard to PCR as a whole part for the hairpin structure.When this chromoprotein is used as a reporter gene, you must consider that whether the upstream has a RBS and whether the downstream has a terminator.


Source

The Star3 system including Target3 and Antisense3 is originated from the T181 strain (Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs.) with certain modification and improvement.

References